Aug 10, 2021 · In this Review, we discuss the design of lipid nanoparticles for mRNA delivery and examine physiological barriers and possible administration routes for lipid nanoparticle–mRNA systems.

Lipid nanoparticles (LNPs), based on ionizable lipids, are the most commonly used messenger RNA (mRNA) delivery vectors and play a crucial role in the research and clinical translation of mRNA therapy.

In this review, we summarize recent site-specific mRNA delivery strategies, including different organ- or tissue-specific LNP after local injection, and organ-specific or cell-specific LNP after intravenous injection. We also provide an outlook on the prospects of mRNA therapy.

Jun 28, 2021 · Lipid nanoparticles (LNPs) have emerged across the pharmaceutical industry as promising vehicles to deliver a variety of therapeutics. Currently in the spotlight as vital components of the COVID-19 mRNA vaccines, LNPs play a key role in effectively protecting and transporting mRNA to cells.

6 days ago · Several years ago, other researchers discovered that adding a branch to the end of LNPs’ normally linear lipid tails dramatically improved mRNA delivery. This discovery prompted Marshall Padilla, a postdoctoral fellow in the Mitchell Lab, to question if it could serve as the key to developing more effective lipids for mRNA delivery. One major ...

Lipid nanoparticles (LNPs) have emerged as the preeminent nonviral drug delivery vehicles for nucleic acid therapeutics, as exemplified by their usage in the mRNA COVID‐19 vaccines. As a safe and highly modular delivery platform, LNPs are attractive for a wide range of applications.

Jan 29, 2024 · With the recent success of lipid nanoparticle (LNP) based SARS-CoV-2 mRNA vaccines, the potential for RNA therapeutics has gained widespread attention. LNPs are promising non-viral delivery...

Dec 7, 2021 · After decades of extensive fundamental studies and clinical trials, lipid nanoparticles (LNPs) have demonstrated effective mRNA delivery such as the Moderna and Pfizer-BioNTech vaccines fighting against COVID-19.

6 days ago · a, Survival curve graph of naive C57BL/6 (‘Black-6’) mice i.v. injected with 1 mg kg −1 of either mRNA-LNP or pDNA-LNP shows 100% mortality in mice treated with pDNA-LNPs compared with 0% in ...

Jun 14, 2022 · In the last decade, the development of messenger RNA (mRNA) therapeutics by lipid nanoparticles (LNP) leads to facilitate clinical trial recruitment, which improves the efficacy of treatment modality to a large extent.

Sep 22, 2024 · Lipid nanoparticles (LNPs), based on ionizable lipids, are the most commonly used messenger RNA (mRNA) delivery vectors and play a crucial role in the research and clinical translation of mRNA therapy.

Aug 1, 2024 · To overcome the biological barriers of mRNA, lipid nanoparticles (LNPs) have emerged as promising mRNA-packing nanotechnology, gaining revolutionary advances attributed to the widespread applications of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mRNA-LNP vaccines [6], [7].

Jan 15, 2025 · In this study, we have compared the difference between two SARS-CoV-2 receptor-binding domain (RBD) mRNA-Lipid nanoparticle (LNP) vaccines prepared from two different ionizable cationic lipids: ALC-0315 and MC3.

Jan 30, 2025 · Therapeutic drugs and multivalent vaccines based on the delivery of mRNA via lipid nanoparticle (LNP) technologies are expected to dominate the biopharmaceutical industry landscape in the coming years. Many of these innovative therapies include several nucleic acid components (e.g., nuclease mRNA and guide RNA) posing unique analytical challenges when …

Rapid developments in the coronavirus disease 2019 (COVID-19) mRNA vaccine showcased the power of lipid nanoparticle (LNP) delivery systems in fighting infectious diseases. In addition, mRNA therapeutics are also in development for cancer immunotherapy.

Jan 13, 2025 · The LNP formulation used in Moderna’s Spikevax mRNA vaccine (LNP-M) demonstrated a stable nanoparticle structure, high expression efficiency, and low toxicity. Notably, a DNA vaccine encoding the spike protein, delivered via LNP-M, induced stronger antigen-specific antibody and T cell immune responses compared to electroporation.

Dec 27, 2024 · With the approval of the first RNA-based drug using LNP technology, the cationic lipid MC3 has successfully reignited interest in RNA delivery. Various LNP components have been developed, accelerating the clinical development of other LNP-based RNA therapies, particularly mRNA COVID-19 vaccines.

Jan 23, 2023 · The mRNA-LNP vaccine has several advantages, such as rapid antigen design, the potential for quick, inexpensive, and scalable manufacturing, and the induction of strong humoral and cellular...

Aug 23, 2024 · Collectively, RD-LNPs delivered mRNA more efficiently to the liver, lung, spleen and brains of mice and to haematopoietic stem and progenitor cells in vitro than conventional LNPs. These...

3 days ago · This study aimed to devise a novel LNP by substituting cholesterol with MP (MP-LNP) and encapsulating C3-mRNA (MP-LNP-C3) for combined treatment of SCI. Mice subjected to clip-induced SCI were locally administered a single dose of MP-LNP-C3. Once internalized, MP can diffuse out to bind its receptor and significantly reduce the inflammatory ...

Jan 11, 2023 · Overall, we have developed peptide-conjugated LNPs that can enable mRNA delivery to the neural retina, expanding the utility of LNP-mRNA therapies for inherited blindness. Lipid nanoparticles (LNPs) are the most clinically advanced nonviral platform for mRNA delivery.

Novel LNP-mRNA vaccines and treatments hold tremendous potential for future development, and with improved targeting strategies as described herein, the number of both preclinical and clinical studies is sure to multiply in the coming years.

Aug 21, 2024 · mRNA作为开发新型治疗药物的钥匙，有着近乎无限的应用前景，除了传染病领域，mRNA技术还能在肿瘤， 蛋白替代疗法 等诸多赛道得到运用。 mRNA-脂质纳米颗粒（ mRNA-LNP ）药物已在飞速发展，引发一场医学革命。 在未来，几乎任何疾病都将可以使用基于mRNA的 基因疗法 进行治疗。

Sep 23, 2022 · Lipid nanoparticles (LNPs) are effective vehicles to deliver mRNA vaccines and therapeutics. It has been challenging to assess mRNA packaging characteristics in LNPs, including payload...

Feb 24, 2024 · The mRNA-LNP vaccine has received much attention during the COVID-19 pandemic since it served as the basis of the most widely used SARS-CoV-2 vaccines in Western countries. Based on early clinical trial data, these vaccines …