The second-step PCR of the RT-nPCR was carried out by using the TaKaRa Premix Taq™ (Ex Taq™ Version 2.0 ... Two target CMNV-specific RNA-dependent RNA polymerase (RdRp) gene fragments of 619 and 413 ...

Bloodstain samples of 279 unrelated healthy Hainan Li individuals were collected after receiving their informed consents. The experiment was conducted in accordance with the guidelines of humane and ...

1x PCR buffer and 1.5 U of Taq DNA polymerase. PCR amplifications consisted of 30 cycles at 94°C for 1 min, annealing temperature specific for each primer for 1 min (Table 1) and 72°C for 2 min in a ...