Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 44 , 283–292 (1986) An analysis of 5'-noncoding sequences from 699 ...

Professor Hirohide Saito and his research team have developed a new microRNA-responsive hybrid mRNA switch, enabling precise, ...

Additionally, it dramatically increased antibody titer by up to 128-fold in vivo, compared to the codon-optimization benchmark mRNA sequence H.

To overcome the inherent challenge of translation termination interference caused by stop codon reprogramming in mammalian ...

The tRNA translates the mRNA codon sequence into a growing chain of amino acids that will become a protein. A codon is a sequence of three consecutive DNA or RNA nucleotides that corresponds with ...

The team then redesigned mRNA sequences to avoid these ‘off-target’ effects, by correcting the error-prone genetic sequences in the synthetic mRNA. This produced the intended protein.” At issue is a ...

mRNA display is an entire in vitro selection method that takes advantage of cell-free translation systems for the generation of polypeptide sequences. Both the expression and the selection are ...