Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 44 , 283–292 (1986) An analysis of 5'-noncoding sequences from 699 ...

Additionally, it dramatically increased antibody titer by up to 128-fold in vivo, compared to the codon-optimization benchmark mRNA sequence H.

The team then redesigned mRNA sequences to avoid these ‘off-target’ effects, by correcting the error-prone genetic sequences in the synthetic mRNA. This produced the intended protein.” At issue is a ...

Ribosomes translocate along the mRNA strands one codon at a time. Codons are three nucleotide sequences in RNA that correspond to an amino acid; each codon binds to a tRNA molecule carrying the ...

mRNA display is an entire in vitro selection method that takes advantage of cell-free translation systems for the generation of polypeptide sequences. Both the expression and the selection are ...