“Products created during setup decrease the efficiency of PCR,” says Joseph Donnenhoffer of Roche. The company's FastStart Taq polymerase has a one-base deletion that renders the enzyme ...

This manuscript characterizes a mutated clone of RNA polymerase I in yeast, referred to as SuperPol, to understand the mechanisms of RNA polymerase I elongation and termination. The authors present ...

The premix was reverse-transcribed using an Evo-M-MLV (AG11706) and SYBR Green Pro Taq HS Pre-Mix qPCR Kit (AG11701) for reverse transcription and quantitative fluorescence PCR. For the quantitative ...

Polymerase chain reaction (PCR) was performed in a 50-μL reaction mixture containing 25 μL of 2 × Premix Taq (Takara Biotechnology, Dalian Co. Ltd., China), 1 μL of each primer (10 μM), and 3 μL of ...

DNA detection at baseline was associated with worse recurrence-free, overall survival in BRAFV600-mutant stage III melanoma.

The Applied Biosystems™ QuantStudio™ Real-Time PCR (qPCR) instruments build on innovations developed by Thermo Fisher Scientific™ chemists and engineers. The Applied Biosystems™ VeriFlex™ system, ...

Sorghum millet is seen as the crop plant of the future: It builds up a particularly high amount of biomass and thrives in harsh conditions. Certain varieties even produce more sugar on salty soils.

Flowering time is a crucial step in the adaptation of annual crops such as oat. The main objectives of this work were to characterize flowering time in oat genotypes of different origins in field ...