“Products created during setup decrease the efficiency of PCR,” says Joseph Donnenhoffer of Roche. The company's FastStart Taq polymerase has a one-base deletion that renders the enzyme ...

Since PCR's advent, scientists have developed filters to manage ... more tightly around the DNA in the active site,” said McLaren. They aimed to improve Taq polymerase by incorporating this feature ...

Note, the pH is 9.2 (the optimal for long PCR) without adjustment; some vectors may require pH 7.2. 10-40 dNTP mix: 10 mM each dNTP, 40 mM MgCl 2; dissolve the dry form of each dNTP in water to ...