In fact, STED and PALM/STORM, as well as the more recent super-resolution fluorescence microscopes, are all based on this ON/OFF principle. Transferring nearby molecules briefly from a fluorescent ...

It has been argued that current super-resolution methods should be viewed in a unified way as applications of a single idea; the switching of molecules between an on and off state 29. We have ...

Despite the remarkable advances in super-resolution microscopy, several challenges remain. One of the main challenges is the trade-off between spatial resolution, temporal resolution, and imaging ...