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PP7-tagged pre-mRNA was generated by subcloning a primer extension product of the 24-nt PP7 binding site into either the KpnI-SacI sites (5′ exon tag) or the BamHI site ...
The proteins MCP and PCP fused to EGFP or mCherry, respectively, were expressed from lentiviral plasmids. dCas9 allows binding of sgRNAs to their target DNAs and sgRNAs containing MS2 or PP7 stem ...
The first round of translation displaces NLS-PCP-GFP from the oskTRICK transcript as the ribosome traverses the coding region that contains the PP7 binding sites, resulting in the loss of NLS-PCP ...
The first round of translation displaces NLS-PCP-GFP from the oskTRICK transcript as the ribosome traverses the coding region that contains the PP7 binding sites, resulting in the loss of NLS-PCP ...
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