Rational design is the classical protein engineering method that involves site directed mutagenesis. 7 Scientists perform specific point mutations via insertions or deletions in the coding sequence ...

Amino acids have different side chains ... form long linear or fibrillar aggregates known as amyloid deposits. But how can a protein change so radically by folding differently, if the sequence ...

If the leader is long, it may contain regulatory sequences ... polypeptide chain in the appropriate order. At this point, translation must be terminated, and the nascent protein must be released ...